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KMID : 0613820180280060681
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Journal of Life Science
2018 Volume.28 No. 6 p.681 ~ p.687
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Whitening Effect and Skin Regeneration Effect of Red Sea Cucumber Extract
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Jeon Mi-Ji
Kim Eun-Ji
Kim Ga-Yeon
Lee Seung-Je
Jung In-Cheol
Kim Sang-Yong
Kim Young-Min
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Abstract
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Recently, several researchers have been developing cosmetics from natural ingredients for skin whitening and anti-aging products. The red sea cucumber (RSC), Apostichopus japonicas, is a species of sea cucumber in the family stichopodiae, which is widely distributed in China, Japan, and Korea. To use Red Sea Cucumber as a cosmetic ingredient, its inhibitory effects on melanogenesis and the anti-aging effects of RSC extracts were investigated. First, a tyrosinase activity assay was performed, which showed that RSC inhibited tyrosinase activity at a concentration of 200 μg/ml. An MTT assay was carried out to evaluate cell toxicity, and the results showed that RSC extract has no cytotoxicity in HaCaT cells. Furthermore, the mRNA expression levels of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), microphthalmia-associated transcription factor (MITF), and matrix metalloproteinase (MMPs) genes treated with RSC extract in B16F10 and HaCaT cells decreased. Moreover, a wound-healing assay was performed to identify the cell regeneration effect of RSC extracts. Also, a skin turnover effect was confirmed by creating a three-dimensional cell culture with HaCaT and human fibroblasts. Altogether, the results suggested that Red Sea Cucumber may possess a high ability to induce whitening and anti-wrinkle effects as a cosmeceutical ingredient.
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KEYWORD
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Anti-melanogenesis, anti-wrinkle, B16F10 cells, organotypic culture, Red Sea Cucumber
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